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>Corresponding Author : Michel Leclerc

>Article Type : Research Article

>Volume : 2   |   Issue : 1

>Received Date : 11 January, 2022

>Accepted Date : 20 January, 2022

>Published Date : 23 January, 2022

>DOI : https://doi.org/10.54289/JVVD2200102

Citation & Copyright

>Citation : : Leclerc M. (2022) Hla-E Gene from Ophiocomina Nigra (Echinodermata-Invertebrates). Bioinformatics Data. J Virol Viral Dis 2(1): doi https://doi.org/10.54289/JVVD2200102  

>Copyright : © 2022 Leclerc M. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Journal of Virology and Viral Diseases

Research Article | Open Access | Full Text

Hla-E Gene from Ophiocomina Nigra (Echinodermata-Invertebrates). Bioinformatics Data
Michel Leclerc*

Immunology of Invertebrates, 556 Rue Isabelle Romée, 45640 Sandillon France

*Corresponding author: Michel Leclerc, Immunology of Invertebrates, 556 Rue Isabelle Romée, 45640 Sandillon France

Abstract

HLA-E gene from MHC system has been recently discovered, in our laboratory, in Invertebrates. Blasts were performed against different species to find or not homologies. Results were given in the precedent communication.

Introduction:

In 2020, we discovered for the first time, MHC genes in Invertebrates and particularly in Echinodermata [1, 2]. More recently, in 2022 a biosynthesis of HLA-E (ClassI, MHC) gene from O.nigra was performed [3]. The aim of this work is to analyse HLA-E DNA sequence.

Material and Methods:

Starting material: dna sequence of HLA-E transcriptome:
TGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACAGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCGGGCGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAAA

Results and conclusion

1. Blastn original sequence:
Database: Standard databases were used
We also optimize for: Highly similar sequences (megablast)
We recall that Molecule type is dna
Its query length is 281
We find more than 100 sequences producing significant alignments
First results appear in the table below:

  Table 1

Description Scientific name Max score Total score Query cover E. Value Per. Ident Acc Len Accession
Pan troglodytes chromosomes unknow clone CH251-153M19, complete sequence Pan troglodytes 514 9982 100% 2e-141 99.64% 184578 AC280142.1
Pan troglodytes BAC clone CH251-501A13 from chromosomes unknow, complete sequence Pan troglodytes 514 10636 100% 2e-141 99.64% 181275 AC185293.4
Homo sapiens clone RP11-92L24 from 2 from chromosomes unknow, complete sequence Homo sapiens 514 2329 100% 9e-141 99.64% 137248 AC019051.8
Eukaryotic synthetic construct chromosome 13 Homo sapiens 508 1.314e+06 100% 9e-140 99.29% 96089878 CP034516.1

2. Blastn original sequence: The Molecule type is again dna with a query length of 281
The Database which is used consists in: Non-redundant protein sequences (nr)
We obtain more than 100 sequences producing significant aligments
The table is recapitulated as following in Table 2:

  Table 2

Scientific name Max score Total score Query cover E. Value Per. Ident Acc Len Accession
Description
Hypothetical protein EGM_09670 [Macaca fascicularis] Macaca fascicularis 149 149 91% 1e-44 91.86% 89 EHH59533.1
hCG2030582 [Homo sapiens] Homo sapiens 135 135 90% 5e-39 83.53% 102 EAW48014.1
Low quality protein: histone demethylase UTY [Chlorocebus sabaeus] Chlorocebus sabaeus 129 219 91% 7e-36 87.50% 166 XP_037863302.1
hypothetical protein CRU82_14500 [Staphylococcus aureus] Staphylococcus aureus 124 124 71% 7e-35 92.54% 72 PGG78133.1

Conclusion:

Results summarized in the 2 tables show homologies between the Ophiocomina nigra HLA-E gene and various proteins issued from Staphylococcus aureus to human Chromosome 13 which is sometimes implicated in human trisomy We note also a strong homology with Macaca fascicularis.: 91,86% of identity.
Mainly we retain that O.nigra HLA-E gene exists in” its own right” and in its amplification in plasmid [3].

References:

  1. Leclerc M. (2020) Evidence of MHC Class I and Class II Genes in Echinodermata. 2(1): 59-61. [Ref.]
  2. Leclerc M. (2021) Biosynthesis « De Novo » of the Ophuirid Ophiocomina Nigra Igkappa Gene.1(1): 1-4. [Ref.]
  3. Leclerc M. (2022) Ophuirid Ophiocomina Nigra HLA-E Gene Synthesis in PUC-GW-KAN Plasmid or HLA-E Echinodermata Gene Biosynthesis « De Novo » in E. Coli Sensu Lato Plasmid. J Virol Viral Dis 2(1). [Ref.]
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